Vitis vinifera (seed)

Nomenclature

Vitis vinifera L.   Vitaceae  
Standardized common name (English): grape  
Ayurvedic name(s): draksha

Botanical Voucher Specimen

Source: MOBOT, Tropicos.org

Microscopic Characteristics

Raphides containing idioblast of needles of calcium oxalate observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories	Thick layer of sclereids observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories

High Performance Thin Layer Chromatographic Identification

Vitis vinifera HPTLC ID - Vanillin Sulfuric Reagent UV 365 nm Grape (seed) (Vitis vinifera) Lane Assignments Lanes, from left to right (Track, Volume, Sample): 3 μL Catechin ~0.1% in Methanol 3 μL Vitis vinifera-1 (peel) 3 μL Vitis vinifera-2 (seed) 3 μL Vitis vinifera-3 (seed) 3 μL Vitis vinifera-3 (seed) 3 μL Vitis vinifera-4 (seed) 3 μL Vitis vinifera-5 (seed) 3 μL Epicatechin gallate ~0.1% in Methanol Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.  Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates  Mobile Phase chloroform: ethyl formate: formic acid [6/3/1]  Sample Preparation Method 0.3 g + 3ml 70% grain EtOH sonicated + heated @ 50° C ~ 1 hr  Detection Method Vanillin/H2SO4 spray reagent -> 110° C 5 min -> UV 365 nm  Reference see American Herbal Pharmacopoeia & Therapeutic Compendium Source: Elan M. Sudberg, Alkemist Laboratories