Medicago sativa (leaf)

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Revision as of 18:55, 14 March 2014

Contents

Nomenclature

Medicago sativa L.   Fabaceae  
Standardized common name (English): alfalfa

Introduction

Macroscopic Entries

Microscopic Entries

Long unicellular trichome showing warted exine observed at 400x with Acidified Chloral Hydrate Glycerol Solution.cellular structures identified in this botanical specimen are the long unicellular trichome showing warted exine and the prisms of calcium oxalate when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [1]

AP-LOGO-Laboratories Crop - Copy.jpg
Alfalfa Alkemist Laboratories.jpg


HPTLC Entries

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(thumbnail)
Medicago sativa L. HPTLC ID - Ninhydrin Reagent, Visible Light

Alfalfa (leaf) (Medicago sativa L.)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 1 μL Arginine ~0.1% in CH3OH
  2. 3 μL ‘‘Medicago sativa’’ L. (leaf)
  3. 3 μL ‘‘Medicago sativa’’ L. (herb)
  4. 3 μL ‘‘Medicago sativa’’ L. (leaf)
  5. 3 μL ‘‘Medicago sativa’’ L. (leaf)
  6. 3 μL ‘‘Medicago sativa’’ L. (leaf)
  7. 3 μL ‘‘Medicago sativa’’ L. (leaf)
  8. 3 μL Canavanine ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase n-butanol: pyridine: AcCOOH: H2O [5/1.25/1.25/2] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr. 

Detection Method Ninhydrin Reagent -> visible light 

Reference see Phytochemical Methods, Harborne, J.B., 1998, p. 198


Source: Elan M. Sudberg, Alkemist Laboratories [2]


Other Points of Interest


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