Triticum aestivum (leaf)

From AHPA Botanical Identity References Compendium
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=Introduction=
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{{DISPLAYTITLE:''Triticum aestivum'' (leaf) }} {{askbox|herb=''Triticum aestivum''}}
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=Macroscopic Entries=
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=Nomenclature=
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=Microscopic Entries=
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{{nomenclature | binomial=Triticum aestivum
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=HPTLC Entries=
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|authority=L.
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=Other Points of Interest=
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|family=Poeceae
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|scn=wheat
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|syn=
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|ayurvedic=
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|pinyin=
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|aka=common wheat
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|notes= }}
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=Botanical Voucher Specimen=
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{{Media      |cat=Voucher
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            | source=MOBOT, Tropicos.org
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            | mainimage=Triticum aestivum Tropicos 100173767.jpg
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            | companyimage=TropicosLogo.gif
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            | companyURL=http://www.tropicos.org/Image/100173767
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            | reference=Tropicos.org. Missouri Botanical Garden. 21 Mar 2014 <http://www.tropicos.org/Image/100173767>
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            | }}
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=Organoleptic Characteristics=
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=Macroscopic Characteristics=
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=Microscopic Characteristics=
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{{Media2 |cat=Microscopy | source=Elan M. Sudberg, Alkemist Laboratories
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            | companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL=http://www.alkemist.com
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            | mainimage=Triticum aestivum - Alkemist Laboratories.jpg
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            | caption1=Unicellular thick walled tapering trichome observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
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| source2=Elan M. Sudberg, Alkemist Laboratories
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            | companyimage2= AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL2=http://www.alkemist.com
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            | description= Wheat Grass (leaf) (''Triticum aestivum'')
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            | image2=Triticum aestivum-1 - Alkemist Laboratories.jpg
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            | caption2=Epidermis showing long thin stomata observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
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            | reference=An Anatomical Atlas of Vegetable Powders, Greenish, H., 1904
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            | }}
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=High Performance Thin Layer Chromatographic Identification=
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{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
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            | description=Wheat grass (grass) (''Triticum aestivum'')
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            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL=http://www.alkemist.com
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            | mainimage=Triticum aestivum - Alkemists Laboratories.jpg
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            | caption1=''Triticum aestivum'' HPTLC ID - Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm
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            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
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            | mobilephase=chloroform: methanol: water [6.4/5/1]
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            | prep=0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr
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            | detection= Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm
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            | lanes= Lanes, from left to right (Track, Volume, Sample):
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# 3 μL Aescin~0.1% in CH3OH
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# 2 μL ''Triticum aestivum''-1 (grass)
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# 4 μL ''Triticum aestivum''-1 (grass)
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# 3 μL ''Triticum aestivum''-2 (grass)
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# 3 μL ''Triticum aestivum''-2 (grass)
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# 3 μL ''Triticum aestivum''-3 (grass)
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# 3 μL ''Triticum aestivum''-4 (grass)
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# 3 μL Aescin~0.1% in CH3OH
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Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.
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            | reference= Method Developed by Alkemists Laboratories
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            | }}
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=Supplementary Information=
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=Sources=
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<references />

Latest revision as of 21:25, 2 June 2014

AHPA recognizes other valuable resources exist regarding the identity of Triticum aestivum.

To submit a suggestion or contribution, please contact Merle Zimmermann.

Contents

Nomenclature

Triticum aestivum L.   Poeceae  
Standardized common name (English): wheat

Botanical Voucher Specimen

bottomright

Triticum aestivum Tropicos 100173767.jpg
Source: MOBOT, Tropicos.org[1]

Organoleptic Characteristics

Macroscopic Characteristics

Microscopic Characteristics

bottomright bottomright

Triticum aestivum - Alkemist Laboratories.jpg
Unicellular thick walled tapering trichome observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[2]

Triticum aestivum-1 - Alkemist Laboratories.jpg
Epidermis showing long thin stomata observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[3]


High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Triticum aestivum HPTLC ID - Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm

Wheat grass (grass) (Triticum aestivum)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Aescin~0.1% in CH3OH
  2. 2 μL Triticum aestivum-1 (grass)
  3. 4 μL Triticum aestivum-1 (grass)
  4. 3 μL Triticum aestivum-2 (grass)
  5. 3 μL Triticum aestivum-2 (grass)
  6. 3 μL Triticum aestivum-3 (grass)
  7. 3 μL Triticum aestivum-4 (grass)
  8. 3 μL Aescin~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase chloroform: methanol: water [6.4/5/1] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see Method Developed by Alkemists Laboratories


Source: Elan M. Sudberg, Alkemist Laboratories [4]


Supplementary Information

Sources

  1. ↑ MOBOT, Tropicos.org http://www.tropicos.org/Image/100173767
  2. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  3. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  4. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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