Citrus paradisi HPTLC ID - 10% Ethanolic H2SO4-> 115° C 15 min -> UV 365 nm

Grapefruit (seed) (Citrus paradisi)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

1. 3 μL Hesperidin, Hesperitin ~0.1% in CH3OH
2. 3 μL Citrus paradisi-1 (seed)
3. 3 μL Citrus paradisi-2 (seed)
4. 3 μL Citrus paradisi-3 (seed)
5. 3 μL Citrus paradisi-3 (seed)
6. 3 μL Citrus paradisi-4 (seed)
7. 3 μL Citrus paradisi-5 (seed)
8. 3 μL Naringin, Naringenin ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase CHCl3: CH3OH: HCOOH: H2O [6/2/1.4/0.1] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicate/heat @ 50° C ~ 1/2 hr 

Detection Method 10% Ethanolic H2SO4-> 115° C 15 min -> UV 365 nm 

Reference see Method Developed by Alkemists Laboratories

Source: Elan M. Sudberg, Alkemist Laboratories ^[1]