Medicago sativa L. HPTLC ID - Ninhydrin Reagent, Visible Light

Alfalfa (leaf) (Medicago sativa L.)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

1. 1 μL Arginine ~0.1% in CH3OH
2. 3 μL ‘‘Medicago sativa’’ L. (leaf)
3. 3 μL ‘‘Medicago sativa’’ L. (herb)
4. 3 μL ‘‘Medicago sativa’’ L. (leaf)
5. 3 μL ‘‘Medicago sativa’’ L. (leaf)
6. 3 μL ‘‘Medicago sativa’’ L. (leaf)
7. 3 μL ‘‘Medicago sativa’’ L. (leaf)
8. 3 μL Canavanine ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase n-butanol: pyridine: AcCOOH: H2O [5/1.25/1.25/2] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr. 

Detection Method Ninhydrin Reagent -> visible light 

Reference see Phytochemical Methods, Harborne, J.B., 1998, p. 198

Source: Elan M. Sudberg, Alkemist Laboratories ^[2]