Arnebia root, zi cao (root) HPTLC ID - UV 366 nm

Arnebia root, zi cao (root) (Arnebia euchroma or Arnebia guttata)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

1. 3 µL Arnebia root 1
2. 5 µL Arnebia root 1
3. 7 µL Arnebia root 1
4. 7 µL Arnebia root 2
5. 7 µL Arnebia root 3
6. 3 µL CAMAG test dye mixture III
7. 7 µL Arnebia root 4 (old sample)
8. 7 µL Yunnan onosma root
9. 7 µL Tibetan onosma root 

Reference Sample(s) Reference: Dilute CAMAG test dye mixture III 1:10 with toluene. 

Stationary Phase Stationary phase, i.e. Silica gel 60, RP-18 

Mobile Phase acetone, 5% aqueous formic acid 8:2 (v/v) 

Sample Preparation Method Sample: Mix 500 mg of powdered sample with 5 mL of methanol and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions. 

Detection Method Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33% 

Other Notes Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.

System suitability test (under white RT): CAMAG test dye mixture III: A blue zone: at Rf ~ 0.28 and an orange red double zone at Rf ~ 0.48.

Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. Under UV 366 nm the chromatogram of the test solution shows a blue zone right above the application position, a diffuse red zone at Rf ~ 0.35, above it another blue, a red and a purple zone and a characteristic blue zone at Rf ~ 0.71. Under white RT there is an intense and diffuse purple zone at Rf ~ 0.35. Above it there is a purple zone at Rf ~ 0.50 and at Rf ~ 0.60. A blue zone is visible at Rf ~ 0.67.

Test for adulteration: Under white RT no zones are seen at Rf ~ 0.60 (black arrow) and Rf ~ 0.67 (red arrow) (Yunnan onosma root, Tibetan onosma root, or low quality/old Arnebia root).

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  Arnebia root, zi cao (root) HPTLC ID - UV 366 nm

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  Arnebia root, zi cao (root) HPTLC ID - White RT

Source: HPTLC Association ^[2]