Citrus paradisi (seed)

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Revision as of 21:56, 15 March 2014


Contents

Nomenclature

Citrus x paradisi Macfad.   Rutaceae  
Standardized common name (English): grapefruit

Botanical Voucher Specimen

Organoleptic Characteristics

Macroscopic Characteristics

Microscopic Characteristics

High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Citrus paradisi HPTLC ID - 10% Ethanolic H2SO4-> 115° C 15 min -> UV 365 nm

Grapefruit (seed) (Citrus paradisi)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Hesperidin, Hesperitin ~0.1% in CH3OH
  2. 3 μL Citrus paradisi-1 (seed)
  3. 3 μL Citrus paradisi-2 (seed)
  4. 3 μL Citrus paradisi-3 (seed)
  5. 3 μL Citrus paradisi-3 (seed)
  6. 3 μL Citrus paradisi-4 (seed)
  7. 3 μL Citrus paradisi-5 (seed)
  8. 3 μL Naringin, Naringenin ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase CHCl3: CH3OH: HCOOH: H2O [6/2/1.4/0.1] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicate/heat @ 50° C ~ 1/2 hr 

Detection Method 10% Ethanolic H2SO4-> 115° C 15 min -> UV 365 nm 

Reference see Method Developed by Alkemists Laboratories


Source: Elan M. Sudberg, Alkemist Laboratories [1]

Supplementary Information

Sources

  1. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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