Citrus paradisi (seed)

Nomenclature

Citrus x paradisi Macfad.   Rutaceae  
Standardized common name (English): grapefruit

Botanical Voucher Specimen

Citrus paradisi Macfad. Source: Images courtesy of the C.V. Starr Virtual Herbarium of the New York Botanical Garden

High Performance Thin Layer Chromatographic Identification

Citrus paradisi HPTLC ID - 10% Ethanolic H2SO4-> 115° C 15 min -> UV 365 nm Grapefruit (seed) (Citrus paradisi) Lane Assignments Lanes, from left to right (Track, Volume, Sample): 3 μL Hesperidin, Hesperitin ~0.1% in CH3OH 3 μL Citrus paradisi-1 (seed) 3 μL Citrus paradisi-2 (seed) 3 μL Citrus paradisi-3 (seed) 3 μL Citrus paradisi-3 (seed) 3 μL Citrus paradisi-4 (seed) 3 μL Citrus paradisi-5 (seed) 3 μL Naringin, Naringenin ~0.1% in CH3OH Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.  Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates  Mobile Phase CHCl3: CH3OH: HCOOH: H2O [6/2/1.4/0.1]  Sample Preparation Method 0.3 g + 3 ml CH3OH sonicate/heat @ 50° C ~ 1/2 hr  Detection Method 10% Ethanolic H2SO4-> 115° C 15 min -> UV 365 nm  Reference see Method Developed by Alkemists Laboratories Source: Elan M. Sudberg, Alkemist Laboratories

Supplementary Information

ABC Botanical Adulterants Prevention Program Laboratory Guidance

Grapefruit (seed extract) (Citrus paradisi) 

General Characteristics This ABC BAPP Laboratory Guidance Document presents an overview and comparison of various analytical technologies and methods used to differentiate between authentic grapefruit seed extract ingredients and non-authentic materials, with particular discussion of synthetic microbicidal compounds identified in non-authentic materials, authentic grapefruit seed extract components, and a detailed literature review of methods (including sample characterization via TLC, HPLC-UV, HPLC-MS, GC-MS, and others).

Source: ABC, Botanical Adulterants Prevention Program