Euphrasia stricta (aerial parts)

From AHPA Botanical Identity References Compendium
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=HPTLC Entries=
 
=HPTLC Entries=
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{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
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            | description=Eyebright (aerial parts) (''Euphrasia officinalis'')
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            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL=http://www.alkemist.com
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            | mainimage=Euphrasia_officinalis_-_Alkemists_Laboratories.jpg
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            | caption1=''Euphrasia officinalis'' HPTLC ID - Vanillin/Sulfuric Acid Reagent UV 365 nm
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            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
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            | mobilephase=chloroform: methanol: water [6/4/0.4]
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            | prep=0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr
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            | detection=Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm
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            | lanes= Lanes, from left to right (Track, Volume, Sample):
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# 2 μL Aucubin ~0.1% in CH3OH
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# 3 μL ''Euphrasia officinalis''-1 (aerial parts)
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# 3 μL ''Euphrasia officinalis''-2 (aerial parts)
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# 3 μL ''Euphrasia officinalis''-3 (aerial parts)
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# 3 μL ''Euphrasia officinalis''-3 (aerial parts)
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# 3 μL ''Euphrasia officinalis''-4 (aerial parts)
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# 3 μL ''Euphrasia officinalis''-5 (aerial parts)
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# 2 μL Catalpol ~0.1% in CH3OH
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 +
Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.
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            | reference=Herbal Drugs and Phytopharmaceuticals, Max Wichtl, 3rd ed., 2004
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            | }}
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 +
 
=Other Points of Interest=
 
=Other Points of Interest=
 
[[Category:blank]]
 
[[Category:blank]]

Revision as of 23:33, 22 October 2012

Contents

Introduction

Macroscopic Entries

Microscopic Entries

Red pigmented fragment of the anther as a result of the reaction with chloral hydrate observed at 400x with Acidified Chloral Hydrate Glycerol Solution.cellular structures identified in this botanical specimen are the red pigmented fragment of the anther as a result of the reaction with chloral hydrate and the thick-walled trichome showing rough cuticle when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [1]

AP-LOGO-Laboratories Crop - Copy.jpg
Eyebright-1.jpg


HPTLC Entries

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Euphrasia officinalis HPTLC ID - Vanillin/Sulfuric Acid Reagent UV 365 nm

Eyebright (aerial parts) (Euphrasia officinalis)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 2 μL Aucubin ~0.1% in CH3OH
  2. 3 μL Euphrasia officinalis-1 (aerial parts)
  3. 3 μL Euphrasia officinalis-2 (aerial parts)
  4. 3 μL Euphrasia officinalis-3 (aerial parts)
  5. 3 μL Euphrasia officinalis-3 (aerial parts)
  6. 3 μL Euphrasia officinalis-4 (aerial parts)
  7. 3 μL Euphrasia officinalis-5 (aerial parts)
  8. 2 μL Catalpol ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase chloroform: methanol: water [6/4/0.4] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see Herbal Drugs and Phytopharmaceuticals, Max Wichtl, 3rd ed., 2004


Source: Elan M. Sudberg, Alkemist Laboratories [2]


Other Points of Interest


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