Euphrasia stricta (aerial parts)

From AHPA Botanical Identity References Compendium
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{{DISPLAYTITLE:''Euphrasia officinalis'' (aerial parts) }}
 
=Nomenclature=
 
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{{nomenclature | binomial=Euphrasia stricta
 
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=Macroscopic Entries=
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=Botanical Voucher Specimen=
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=Organoleptic Characteristics=
  
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=Macroscopic Characteristics=
 
{{Macroscopy | source=United States Dispensatory (1918)
 
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=Microscopic Characteristics=
 
{{Microscopy | source=Elan M. Sudberg, Alkemist Laboratories
 
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=HPTLC Entries=
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=High Performance Thin Layer Chromatographic Identification=
 
{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
 
{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
 
             | description=Eyebright (aerial parts) (''Euphrasia officinalis'')
 
             | description=Eyebright (aerial parts) (''Euphrasia officinalis'')
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Revision as of 23:22, 15 March 2014

Contents

Nomenclature

Euphrasia stricta J.P. Wolff ex J.F. Lehm.   Scrophulariaceae  
Standardized common name (English): eyebright

Botanical Voucher Specimen

Organoleptic Characteristics

Macroscopic Characteristics

E. officinalis is a small annual plant

without odor, and of a bitterish, astringent taste.

Source: United States Dispensatory (1918) [1]


Microscopic Characteristics

Red pigmented fragment of the anther as a result of the reaction with chloral hydrate observed at 400x with Acidified Chloral Hydrate Glycerol Solution.cellular structures identified in this botanical specimen are the red pigmented fragment of the anther as a result of the reaction with chloral hydrate and the thick-walled trichome showing rough cuticle when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [2]

AP-LOGO-Laboratories Crop - Copy.jpg
Eyebright-1.jpg


High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Euphrasia officinalis HPTLC ID - Vanillin/Sulfuric Acid Reagent UV 365 nm

Eyebright (aerial parts) (Euphrasia officinalis)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 2 μL Aucubin ~0.1% in CH3OH
  2. 3 μL Euphrasia officinalis-1 (aerial parts)
  3. 3 μL Euphrasia officinalis-2 (aerial parts)
  4. 3 μL Euphrasia officinalis-3 (aerial parts)
  5. 3 μL Euphrasia officinalis-3 (aerial parts)
  6. 3 μL Euphrasia officinalis-4 (aerial parts)
  7. 3 μL Euphrasia officinalis-5 (aerial parts)
  8. 2 μL Catalpol ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase chloroform: methanol: water [6/4/0.4] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see Herbal Drugs and Phytopharmaceuticals, Max Wichtl, 3rd ed., 2004


Source: Elan M. Sudberg, Alkemist Laboratories [3]


Supplementary Information

Sources

  1. United States Dispensatory (1918)
  2. Elan M. Sudberg, Alkemist Laboratories http://www.Alkemist.com
  3. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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