Euphrasia stricta (aerial parts)

Microscopic Entries

Red pigmented fragment of the anther as a result of the reaction with chloral hydrate observed at 400x with Acidified Chloral Hydrate Glycerol Solution.cellular structures identified in this botanical specimen are the red pigmented fragment of the anther as a result of the reaction with chloral hydrate and the thick-walled trichome showing rough cuticle when observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories

HPTLC Entries

Euphrasia officinalis HPTLC ID - Vanillin/Sulfuric Acid Reagent UV 365 nm Eyebright (aerial parts) (Euphrasia officinalis) Lane Assignments Lanes, from left to right (Track, Volume, Sample): 2 μL Aucubin ~0.1% in CH3OH 3 μL Euphrasia officinalis-1 (aerial parts) 3 μL Euphrasia officinalis-2 (aerial parts) 3 μL Euphrasia officinalis-3 (aerial parts) 3 μL Euphrasia officinalis-3 (aerial parts) 3 μL Euphrasia officinalis-4 (aerial parts) 3 μL Euphrasia officinalis-5 (aerial parts) 2 μL Catalpol ~0.1% in CH3OH Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.  Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates  Mobile Phase chloroform: methanol: water [6/4/0.4]  Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr  Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm  Reference see Herbal Drugs and Phytopharmaceuticals, Max Wichtl, 3rd ed., 2004 Source: Elan M. Sudberg, Alkemist Laboratories