Euphrasia stricta (aerial parts)

Nomenclature

Euphrasia stricta J.P. Wolff ex J.F. Lehm.   Scrophulariaceae  
Standardized common name (English): eyebright

Organoleptic Characteristics

E. officinalis is a small annual plant without odor, and of a bitterish, astringent taste. Source: United States Dispensatory (1918)

Microscopic Characteristics

Red pigmented fragment of the anther as a result of the reaction with chloral hydrate observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories	Thick-walled trichome showing rough cuticle observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories

High Performance Thin Layer Chromatographic Identification

Euphrasia officinalis HPTLC ID - Vanillin/Sulfuric Acid Reagent UV 365 nm Eyebright (aerial parts) (Euphrasia officinalis) Lane Assignments Lanes, from left to right (Track, Volume, Sample): 2 μL Aucubin ~0.1% in CH3OH 3 μL Euphrasia officinalis-1 (aerial parts) 3 μL Euphrasia officinalis-2 (aerial parts) 3 μL Euphrasia officinalis-3 (aerial parts) 3 μL Euphrasia officinalis-3 (aerial parts) 3 μL Euphrasia officinalis-4 (aerial parts) 3 μL Euphrasia officinalis-5 (aerial parts) 2 μL Catalpol ~0.1% in CH3OH Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.  Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates  Mobile Phase chloroform: methanol: water [6/4/0.4]  Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr  Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm  Reference see Herbal Drugs and Phytopharmaceuticals, Max Wichtl, 3rd ed., 2004 Source: Elan M. Sudberg, Alkemist Laboratories