Ilex paraguariensis (leaf)

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=HPTLC Entries=
 
=HPTLC Entries=
 +
{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
 +
            | description=Yerba Mate (leaf) (''Ilex paraguariensis'')
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            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL=http://www.alkemist.com
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            | mainimage=Ilex_paraguariensis_-_Alkemists_Laboratories.jpg
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            | caption1=''Ilex paraguariensis'' HPTLC ID - Natural Product Reagent + PEG UV 365 nm
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            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
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            | mobilephase=ethyl acetate: glacial acetic acid: formic acid: water [10/1.1/1.1/2.4]
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            | prep=0.3 g + 3 ml CH3OH sonicate/heat @ 50° C ~ 1/2 hr
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            | detection=Natural Product Reagent + PEG -> UV 365 nm
 +
            | lanes= Lanes, from left to right (Track, Volume, Sample):
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# 2 μL Caffeine ~0.1% in CH3OH
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# 3 μL ''Ilex paraguariensis''-1 (leaf)
 +
# 3 μL ''Ilex paraguariensis''-2 (leaf)
 +
# 3 μL ''Ilex paraguariensis''-3 (leaf)
 +
# 3 μL ''Ilex paraguariensis''-3 (leaf)
 +
# 3 μL ''Ilex paraguariensis''-4 (leaf)
 +
# 3 μL ''Ilex paraguariensis''-5 (leaf)
 +
# 4 μL Theobromine ~0.1% in CH3OH
 +
 +
Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.
 +
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            | reference=Herbal Drugs and Phytopharmaceuticals, Max Wichtl, 3rd ed., 2004
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            | }}
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=Other Points of Interest=
 
=Other Points of Interest=
 
[[Category:blank]]
 
[[Category:blank]]

Revision as of 23:19, 22 October 2012

Contents

Introduction

Macroscopic Entries

Microscopic Entries

Lower epidermis showing actinocytic stomata observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [1]

AP-LOGO-Laboratories Crop - Copy.jpg
Ilex paraguariensis - Alkemist Laboratories.jpg


HPTLC Entries

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Ilex paraguariensis HPTLC ID - Natural Product Reagent + PEG UV 365 nm

Yerba Mate (leaf) (Ilex paraguariensis)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 2 μL Caffeine ~0.1% in CH3OH
  2. 3 μL Ilex paraguariensis-1 (leaf)
  3. 3 μL Ilex paraguariensis-2 (leaf)
  4. 3 μL Ilex paraguariensis-3 (leaf)
  5. 3 μL Ilex paraguariensis-3 (leaf)
  6. 3 μL Ilex paraguariensis-4 (leaf)
  7. 3 μL Ilex paraguariensis-5 (leaf)
  8. 4 μL Theobromine ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: glacial acetic acid: formic acid: water [10/1.1/1.1/2.4] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicate/heat @ 50° C ~ 1/2 hr 

Detection Method Natural Product Reagent + PEG -> UV 365 nm 

Reference see Herbal Drugs and Phytopharmaceuticals, Max Wichtl, 3rd ed., 2004


Source: Elan M. Sudberg, Alkemist Laboratories [2]

Other Points of Interest


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