Ilex paraguariensis (leaf)

Nomenclature

Ilex paraguariensis A. St.-Hil.   Aquifoliaceae  
Standardized common name (English): mate

Microscopic Characteristics

Lower epidermis showing actinocytic stomata observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories

High Performance Thin Layer Chromatographic Identification

Ilex paraguariensis HPTLC ID - Natural Product Reagent + PEG UV 365 nm Yerba Mate (leaf) (Ilex paraguariensis) Lane Assignments Lanes, from left to right (Track, Volume, Sample): 2 μL Caffeine ~0.1% in CH3OH 3 μL Ilex paraguariensis-1 (leaf) 3 μL Ilex paraguariensis-2 (leaf) 3 μL Ilex paraguariensis-3 (leaf) 3 μL Ilex paraguariensis-3 (leaf) 3 μL Ilex paraguariensis-4 (leaf) 3 μL Ilex paraguariensis-5 (leaf) 4 μL Theobromine ~0.1% in CH3OH Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.  Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates  Mobile Phase ethyl acetate: glacial acetic acid: formic acid: water [10/1.1/1.1/2.4]  Sample Preparation Method 0.3 g + 3 ml CH3OH sonicate/heat @ 50° C ~ 1/2 hr  Detection Method Natural Product Reagent + PEG -> UV 365 nm  Reference see Herbal Drugs and Phytopharmaceuticals, Max Wichtl, 3rd ed., 2004 Source: Elan M. Sudberg, Alkemist Laboratories