Medicago sativa (leaf)
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=HPTLC Entries= | =HPTLC Entries= | ||
+ | {{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories | ||
+ | | description=Alfalfa (leaf) (''Medicago sativa'' L.) | ||
+ | | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg | ||
+ | | companyURL=http://www.alkemist.com | ||
+ | | mainimage=Medicago sativa - Alkemist Laboratories.jpg | ||
+ | | caption1=''Medicago sativa'' L. HPTLC ID - Ninhydrin Reagent, Visible Light | ||
+ | | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates | ||
+ | | mobilephase=n-butanol: pyridine: AcCOOH: H2O [5/1.25/1.25/2] | ||
+ | | prep=0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr. | ||
+ | | detection=Ninhydrin Reagent -> visible light | ||
+ | | lanes=Lanes, from left to right (Track, Volume, Sample): | ||
+ | # 1 μL Arginine ~0.1% in CH3OH | ||
+ | # 3 μL ‘‘Medicago sativa’’ L. (leaf) | ||
+ | # 3 μL ‘‘Medicago sativa’’ L. (herb) | ||
+ | # 3 μL ‘‘Medicago sativa’’ L. (leaf) | ||
+ | # 3 μL ‘‘Medicago sativa’’ L. (leaf) | ||
+ | # 3 μL ‘‘Medicago sativa’’ L. (leaf) | ||
+ | # 3 μL ‘‘Medicago sativa’’ L. (leaf) | ||
+ | # 3 μL Canavanine ~0.1% in CH3OH | ||
+ | |||
+ | Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA. | ||
+ | | reference=Phytochemical Methods, Harborne, J.B., 1998, p. 198 | ||
+ | | }} | ||
+ | |||
+ | |||
=Other Points of Interest= | =Other Points of Interest= | ||
[[Category:blank]] | [[Category:blank]] |
Revision as of 20:08, 22 October 2012
Contents |
Introduction
Macroscopic Entries
Microscopic Entries
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HPTLC Entries
Alfalfa (leaf) (Medicago sativa L.) Lane Assignments Lanes, from left to right (Track, Volume, Sample):
Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA. Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates Mobile Phase n-butanol: pyridine: AcCOOH: H2O [5/1.25/1.25/2] Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr. Detection Method Ninhydrin Reagent -> visible light Reference see Phytochemical Methods, Harborne, J.B., 1998, p. 198
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Other Points of Interest
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