Medicago sativa (leaf)
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− | = | + | {{DISPLAYTITLE:''Medicago sativa'' (leaf) }} {{askbox|herb=''Medicago sativa''}} |
− | =Macroscopic | + | =Nomenclature= |
− | =Microscopic | + | {{nomenclature | binomial=Medicago sativa |
− | = | + | |authority=L. |
− | = | + | |family=Fabaceae |
− | + | |scn=alfalfa | |
+ | |syn= | ||
+ | |ayurvedic= | ||
+ | |pinyin= | ||
+ | |aka=lucerne | ||
+ | |notes=}} | ||
+ | |||
+ | =Botanical Voucher Specimen= | ||
+ | |||
+ | {{Media3 |cat=Voucher | ||
+ | |||
+ | | source=MOBOT, Tropicos.org | ||
+ | | mainimage=Medicago_sativa_Tropicos_100136132_(S).jpg | ||
+ | | companyimage=TropicosLogo.gif | ||
+ | | companyURL=http://www.tropicos.org/Image/100136132 | ||
+ | | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100136132> | ||
+ | |||
+ | | companyimage2=Kewlogo.gif | ||
+ | | companyURL2=http://specimens.kew.org/herbarium/K000226899 | ||
+ | | image2=Medicago sativa Kew barcode=K000226899 16978.jpg | ||
+ | | source2=Royal Botanic Gardens, Kew. | ||
+ | |||
+ | | companyimage3=Kewlogo.gif | ||
+ | | companyURL3=http://specimens.kew.org/herbarium/K001122610 | ||
+ | | image3=Medicago sativa Kew barcode=K001122610 708349.jpg | ||
+ | | source3=Royal Botanic Gardens, Kew. | ||
+ | | }} | ||
+ | |||
+ | |||
+ | =Organoleptic Characteristics= | ||
+ | |||
+ | =Macroscopic Characteristics= | ||
+ | {{Media2 |cat=Macroscopy | ||
+ | | source=PlantaPhile | ||
+ | | mainimage=PlantaPhile - 116.jpg | ||
+ | | companyimage=PlantaPhile logo.jpg | ||
+ | | companyURL=http://plantaphile.com/ | ||
+ | | | ||
+ | | source2=PlantaPhile | ||
+ | | image2=PlantaPhile_-_3044.jpg | ||
+ | | companyimage2=PlantaPhile logo.jpg | ||
+ | | companyURL2=http://plantaphile.com/ | ||
+ | | }} | ||
+ | =Microscopic Characteristics= | ||
+ | |||
+ | {{Media2 |cat=Microscopy | source=Elan M. Sudberg, Alkemist Laboratories | ||
+ | | companyimage= AP-LOGO-Laboratories Crop - Copy.jpg | ||
+ | | companyURL=http://www.alkemist.com | ||
+ | | mainimage=Alfalfa Alkemist Laboratories.jpg | ||
+ | | caption1=Long unicellular trichome showing warted exine observed at 400x with Acidified Chloral Hydrate Glycerol Solution. | ||
+ | | source2=Elan M. Sudberg, Alkemist Laboratories | ||
+ | | companyimage2= AP-LOGO-Laboratories Crop - Copy.jpg | ||
+ | | companyURL2=http://www.alkemist.com | ||
+ | | image2=Alfalfa-1 Alkemist Laboratories.jpg | ||
+ | | caption2=Prisms of calcium oxalate observed at 400x with Acidified Chloral Hydrate Glycerol Solution. | ||
+ | | characteristics=cellular structures identified in this botanical specimen are the long unicellular trichome showing warted exine and the prisms of calcium oxalate when observed at 400x with Acidified Chloral Hydrate Glycerol Solution. | ||
+ | | reference=American Herbal Pharmacopoeia & Therapeutic Compendium | ||
+ | | }} | ||
+ | |||
+ | =High Performance Thin Layer Chromatographic Identification= | ||
+ | {{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories | ||
+ | | description=Alfalfa (leaf) (''Medicago sativa'' L.) | ||
+ | | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg | ||
+ | | companyURL=http://www.alkemist.com | ||
+ | | mainimage=Medicago sativa - Alkemist Laboratories.jpg | ||
+ | | caption1=''Medicago sativa'' L. HPTLC ID - Ninhydrin Reagent, Visible Light | ||
+ | | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates | ||
+ | | mobilephase=n-butanol: pyridine: AcCOOH: H2O [5/1.25/1.25/2] | ||
+ | | prep=0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr. | ||
+ | | detection=Ninhydrin Reagent -> visible light | ||
+ | | lanes=Lanes, from left to right (Track, Volume, Sample): | ||
+ | # 1 μL Arginine ~0.1% in CH3OH | ||
+ | # 3 μL ‘‘Medicago sativa’’ L. (leaf) | ||
+ | # 3 μL ‘‘Medicago sativa’’ L. (herb) | ||
+ | # 3 μL ‘‘Medicago sativa’’ L. (leaf) | ||
+ | # 3 μL ‘‘Medicago sativa’’ L. (leaf) | ||
+ | # 3 μL ‘‘Medicago sativa’’ L. (leaf) | ||
+ | # 3 μL ‘‘Medicago sativa’’ L. (leaf) | ||
+ | # 3 μL Canavanine ~0.1% in CH3OH | ||
+ | |||
+ | Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. | ||
+ | | reference=Phytochemical Methods, Harborne, J.B., 1998, p. 198 | ||
+ | | }} | ||
+ | |||
+ | |||
+ | =Supplementary Information= | ||
+ | =Sources= | ||
+ | <references /> |
Latest revision as of 15:14, 26 May 2015
Contents |
Nomenclature
Medicago sativa L. Fabaceae
Standardized common name (English): alfalfa
Botanical Voucher Specimen
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Organoleptic Characteristics
Macroscopic Characteristics
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Microscopic Characteristics
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High Performance Thin Layer Chromatographic Identification
Alfalfa (leaf) (Medicago sativa L.) Lane Assignments Lanes, from left to right (Track, Volume, Sample):
Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates Mobile Phase n-butanol: pyridine: AcCOOH: H2O [5/1.25/1.25/2] Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr. Detection Method Ninhydrin Reagent -> visible light Reference see Phytochemical Methods, Harborne, J.B., 1998, p. 198
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Supplementary Information
Sources
- ↑ MOBOT, Tropicos.org http://www.tropicos.org/Image/100136132
- ↑ Royal Botanic Gardens, Kew. http://specimens.kew.org/herbarium/K000226899
- ↑ Royal Botanic Gardens, Kew. http://specimens.kew.org/herbarium/K001122610
- ↑ PlantaPhile http://plantaphile.com/
- ↑ PlantaPhile http://plantaphile.com/
- ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
- ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
- ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com