Medicago sativa (leaf)

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=Introduction=
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{{DISPLAYTITLE:''Medicago sativa'' (leaf) }} {{askbox|herb=''Medicago sativa''}}
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=Macroscopic Entries=
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=Nomenclature=
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=Microscopic Entries=
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{{nomenclature | binomial=Medicago sativa
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{{Microscopy | source=Elan M. Sudberg, Alkemist Laboratories-American Herbal Pharmacopoeia & Therapeutic Compendium
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|authority=L.
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|family=Fabaceae
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|scn=alfalfa
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|syn=
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|ayurvedic=
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|pinyin=
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|aka=lucerne
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|notes=}}
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=Botanical Voucher Specimen=
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{{Media3      |cat=Voucher
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            | source=MOBOT, Tropicos.org
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            | mainimage=Medicago_sativa_Tropicos_100136132_(S).jpg
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            | companyimage=TropicosLogo.gif
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            | companyURL=http://www.tropicos.org/Image/100136132
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            | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100136132>
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| companyimage2=Kewlogo.gif
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| companyURL2=http://specimens.kew.org/herbarium/K000226899
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| image2=Medicago sativa Kew barcode=K000226899 16978.jpg
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| source2=Royal Botanic Gardens, Kew.
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| companyimage3=Kewlogo.gif
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| companyURL3=http://specimens.kew.org/herbarium/K001122610
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| image3=Medicago sativa Kew barcode=K001122610 708349.jpg
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| source3=Royal Botanic Gardens, Kew.
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            | }}
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=Organoleptic Characteristics=
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=Macroscopic Characteristics=
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{{Media2 |cat=Macroscopy
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| source=PlantaPhile
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            | mainimage=PlantaPhile - 116.jpg
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            | companyimage=PlantaPhile logo.jpg
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            | companyURL=http://plantaphile.com/
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            |
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| source2=PlantaPhile
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            | image2=PlantaPhile_-_3044.jpg
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            | companyimage2=PlantaPhile logo.jpg
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            | companyURL2=http://plantaphile.com/
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            | }}
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=Microscopic Characteristics=
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{{Media2 |cat=Microscopy | source=Elan M. Sudberg, Alkemist Laboratories
 
       | companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
 
       | companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
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       | companyURL=http://www.Alkemist.com
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       | companyURL=http://www.alkemist.com
 
       | mainimage=Alfalfa Alkemist Laboratories.jpg
 
       | mainimage=Alfalfa Alkemist Laboratories.jpg
 
       | caption1=Long unicellular trichome showing warted exine observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
 
       | caption1=Long unicellular trichome showing warted exine observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
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      | description= Alfalfa (leaf) (''Medicago sativa'')
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| source2=Elan M. Sudberg, Alkemist Laboratories
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            | companyimage2= AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL2=http://www.alkemist.com
 
       | image2=Alfalfa-1 Alkemist Laboratories.jpg
 
       | image2=Alfalfa-1 Alkemist Laboratories.jpg
 
       | caption2=Prisms of calcium oxalate observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
 
       | caption2=Prisms of calcium oxalate observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
 
       | characteristics=cellular structures identified in this botanical specimen are the long unicellular trichome showing warted exine and the prisms of calcium oxalate when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
 
       | characteristics=cellular structures identified in this botanical specimen are the long unicellular trichome showing warted exine and the prisms of calcium oxalate when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
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            | reference=American Herbal Pharmacopoeia & Therapeutic Compendium
 
       | }}
 
       | }}
  
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=HPTLC Entries=
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=High Performance Thin Layer Chromatographic Identification=
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=Other Points of Interest=
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{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
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[[Category:blank]]
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            | description=Alfalfa (leaf) (''Medicago sativa'' L.)
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            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL=http://www.alkemist.com
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            | mainimage=Medicago sativa - Alkemist Laboratories.jpg
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            | caption1=''Medicago sativa'' L. HPTLC ID - Ninhydrin Reagent, Visible Light
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            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
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            | mobilephase=n-butanol: pyridine: AcCOOH: H2O [5/1.25/1.25/2]
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            | prep=0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr.
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            | detection=Ninhydrin Reagent -> visible light
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            | lanes=Lanes, from left to right (Track, Volume, Sample):
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# 1 μL Arginine ~0.1% in CH3OH
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# 3 μL ‘‘Medicago sativa’’ L. (leaf)
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# 3 μL ‘‘Medicago sativa’’ L. (herb)
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# 3 μL ‘‘Medicago sativa’’ L. (leaf)
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# 3 μL ‘‘Medicago sativa’’ L. (leaf)
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# 3 μL ‘‘Medicago sativa’’ L. (leaf)
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# 3 μL ‘‘Medicago sativa’’ L. (leaf)
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# 3 μL Canavanine ~0.1% in CH3OH
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Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.
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            | reference=Phytochemical Methods, Harborne, J.B., 1998, p. 198
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            | }}
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=Supplementary Information=
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=Sources=
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<references />

Latest revision as of 15:14, 26 May 2015

AHPA recognizes other valuable resources exist regarding the identity of Medicago sativa.

To submit a suggestion or contribution, please contact Merle Zimmermann.

Contents

Nomenclature

Medicago sativa L.   Fabaceae  
Standardized common name (English): alfalfa

Botanical Voucher Specimen

bottomright bottomright bottomright

Medicago sativa Tropicos 100136132 (S).jpg
Source: MOBOT, Tropicos.org[1]

Medicago sativa Kew barcode=K000226899 16978.jpg
Source: Royal Botanic Gardens, Kew.[2]

Medicago sativa Kew barcode=K001122610 708349.jpg
Source: Royal Botanic Gardens, Kew.[3]


Organoleptic Characteristics

Macroscopic Characteristics

bottomright bottomright

PlantaPhile - 116.jpg
Source: PlantaPhile[4]

PlantaPhile - 3044.jpg
Source: PlantaPhile[5]

Microscopic Characteristics

bottomright bottomright

Alfalfa Alkemist Laboratories.jpg
Long unicellular trichome showing warted exine observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[6]

Alfalfa-1 Alkemist Laboratories.jpg
Prisms of calcium oxalate observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[7]

High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Medicago sativa L. HPTLC ID - Ninhydrin Reagent, Visible Light

Alfalfa (leaf) (Medicago sativa L.)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 1 μL Arginine ~0.1% in CH3OH
  2. 3 μL ‘‘Medicago sativa’’ L. (leaf)
  3. 3 μL ‘‘Medicago sativa’’ L. (herb)
  4. 3 μL ‘‘Medicago sativa’’ L. (leaf)
  5. 3 μL ‘‘Medicago sativa’’ L. (leaf)
  6. 3 μL ‘‘Medicago sativa’’ L. (leaf)
  7. 3 μL ‘‘Medicago sativa’’ L. (leaf)
  8. 3 μL Canavanine ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase n-butanol: pyridine: AcCOOH: H2O [5/1.25/1.25/2] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr. 

Detection Method Ninhydrin Reagent -> visible light 

Reference see Phytochemical Methods, Harborne, J.B., 1998, p. 198


Source: Elan M. Sudberg, Alkemist Laboratories [8]


Supplementary Information

Sources

  1. ↑ MOBOT, Tropicos.org http://www.tropicos.org/Image/100136132
  2. ↑ Royal Botanic Gardens, Kew. http://specimens.kew.org/herbarium/K000226899
  3. ↑ Royal Botanic Gardens, Kew. http://specimens.kew.org/herbarium/K001122610
  4. ↑ PlantaPhile http://plantaphile.com/
  5. ↑ PlantaPhile http://plantaphile.com/
  6. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  7. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  8. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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