Medicago sativa (leaf)

Nomenclature

Medicago sativa L.   Fabaceae  
Standardized common name (English): alfalfa

Microscopic Entries

Long unicellular trichome showing warted exine observed at 400x with Acidified Chloral Hydrate Glycerol Solution.cellular structures identified in this botanical specimen are the long unicellular trichome showing warted exine and the prisms of calcium oxalate when observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories

HPTLC Entries

Medicago sativa L. HPTLC ID - Ninhydrin Reagent, Visible Light Alfalfa (leaf) (Medicago sativa L.) Lane Assignments Lanes, from left to right (Track, Volume, Sample): 1 μL Arginine ~0.1% in CH3OH 3 μL ‘‘Medicago sativa’’ L. (leaf) 3 μL ‘‘Medicago sativa’’ L. (herb) 3 μL ‘‘Medicago sativa’’ L. (leaf) 3 μL ‘‘Medicago sativa’’ L. (leaf) 3 μL ‘‘Medicago sativa’’ L. (leaf) 3 μL ‘‘Medicago sativa’’ L. (leaf) 3 μL Canavanine ~0.1% in CH3OH Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.  Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates  Mobile Phase n-butanol: pyridine: AcCOOH: H2O [5/1.25/1.25/2]  Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr.  Detection Method Ninhydrin Reagent -> visible light  Reference see Phytochemical Methods, Harborne, J.B., 1998, p. 198 Source: Elan M. Sudberg, Alkemist Laboratories