Plantago lanceolata (leaf)

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{{DISPLAYTITLE:''Plantago lanceolata'' (leaf) }} {{askbox|herb=''Plantago lanceolata''}}
 
=Nomenclature=
 
=Nomenclature=
  
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|notes= }}
  
=Macroscopic Entries=
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=Botanical Voucher Specimen=
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{{Media3    | cat=Voucher
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            | source=MOBOT, Tropicos.org
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            | mainimage=Plantago_lanceolata_Tropicos_100010935_(S).jpg
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            | companyimage=TropicosLogo.gif
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            | companyURL=http://www.tropicos.org/Image/100010935
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            | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100010935>
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            | source2=Botanical Voucher Specimen Library, Alkemists Laboratories
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            | image2=Plantago lanceolata RA01405JD A0225.jpg
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            | companyimage2=AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL2=http://www.alkemist.com
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            | source3=Botanical Voucher Specimen Library, Alkemists Laboratories
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            | companyimage3=AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL3=http://www.alkemist.com
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            | image3=Plantago lanceolata RA17404BMX1 A0226.jpg
  
{{Macroscopy | source=Botanical Voucher Specimen Library, Alkemists Laboratories
 
            | mainimage=Plantago lanceolata RA01405JD A0225.jpg
 
            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
 
            | companyURL=http://www.alkemist.com
 
            | caption1=English Plantain Botanical Voucher Specimen - Alkemists Laboratories
 
            | description=English Plantain (''Plantago lanceolata'') Botanical Voucher Specimen
 
| image2=Plantago lanceolata RA17404BMX1 A0226.jpg
 
| caption2=English Plantain (''Plantago lanceolata'') Botanical Voucher Specimen (2/2)
 
 
             | }}
 
             | }}
  
=Microscopic Entries=
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=Organoleptic Characteristics=
{{Microscopy | source=Elan M. Sudberg, Alkemist Laboratories
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=Macroscopic Characteristics=
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=Microscopic Characteristics=
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{{Media2 |cat=Microscopy | source=Elan M. Sudberg, Alkemist Laboratories
 
             | companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
 
             | companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
             | companyURL=http://www.Alkemist.com
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             | companyURL=http://www.alkemist.com
 
             | mainimage=Plantago_lanceolata_-_Alkemist_Laboratories.jpg
 
             | mainimage=Plantago_lanceolata_-_Alkemist_Laboratories.jpg
 
             | caption1=Anomocytic stomate next to a diacytic stomate observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
 
             | caption1=Anomocytic stomate next to a diacytic stomate observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
             | description= Plantain (leaf) (''Plantago lanceolata'')
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| source2=Elan M. Sudberg, Alkemist Laboratories
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            | companyimage2= AP-LOGO-Laboratories Crop - Copy.jpg
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             | companyURL2=http://www.alkemist.com
 
             | image2=Plantago_lanceolata-1_-_Alkemist_Laboratories.jpg
 
             | image2=Plantago_lanceolata-1_-_Alkemist_Laboratories.jpg
 
             | caption2=Sheathed trichome observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
 
             | caption2=Sheathed trichome observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
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             | }}
 
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=HPTLC Entries=
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=High Performance Thin Layer Chromatographic Identification=
 
{{HPTLC | source=HPTLC Association
 
{{HPTLC | source=HPTLC Association
 
             | companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
 
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=Other Points of Interest=
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=Supplementary Information=
 
{{Botanical  | source=AHPA Known Adulterants
 
{{Botanical  | source=AHPA Known Adulterants
 
           | companyimage=AHPA Logo.gif
 
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             | adulterants=''Digitalis lanata'' leaf.
 
             | adulterants=''Digitalis lanata'' leaf.
 
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=Sources=
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<references />

Latest revision as of 21:01, 2 June 2014

AHPA recognizes other valuable resources exist regarding the identity of Plantago lanceolata.

To submit a suggestion or contribution, please contact Merle Zimmermann.

Contents

Nomenclature

Plantago lanceolata L.   Plantaginaceae  
Standardized common name (English): English plantain

Botanical Voucher Specimen

bottomright bottomright bottomright

Plantago lanceolata Tropicos 100010935 (S).jpg
Source: MOBOT, Tropicos.org[1]

Plantago lanceolata RA01405JD A0225.jpg
Source: Botanical Voucher Specimen Library, Alkemists Laboratories[2]

Plantago lanceolata RA17404BMX1 A0226.jpg
Source: Botanical Voucher Specimen Library, Alkemists Laboratories[3]

Organoleptic Characteristics

Macroscopic Characteristics

Microscopic Characteristics

bottomright bottomright

Plantago lanceolata - Alkemist Laboratories.jpg
Anomocytic stomate next to a diacytic stomate observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[4]

Plantago lanceolata-1 - Alkemist Laboratories.jpg
Sheathed trichome observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[5]

High Performance Thin Layer Chromatographic Identification

HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
(thumbnail)
Ribwort plantain (leaf) HPTLC ID - Chloramine - trichloroacetic acid, UV 366 nm

Ribwort plantain (leaf) (Plantago lanceolata)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 4 µl Ribwort plantain leaf 1
  2. 8 µl Ribwort plantain leaf 1
  3. 16 µl Ribwort plantain leaf 1
  4. 4 µl Ribwort plantain leaf 1 (extracted by shaking)
  5. 8 µl Ribwort plantain leaf 1 (extracted by shaking)
  6. 16 µl Ribwort plantain leaf 1 (extracted by shaking)
  7. 5 µl Aucubin
  8. 10 µl Acteoside
  9. 4 µl Grecian foxglove leaf 1
  10. 8 µl Grecian foxglove leaf 1
  11. 16 µl Grecian foxglove leaf 1
  12. 5 µl Grecian foxglove leaf 2
  13. 5 µl Grecian foxglove leaf 3
  14. 5 µl Grecian foxglove leaf 4
  15. 10 µl Greater plantain leaf 

Reference Sample(s) Reference: Dissolve 1 mg of aucubin in 10 mL of methanol. Dissolve 1 mg of acteoside (verbascoside) in 10 mL of methanol 

Stationary Phase Stationary phase, i.e. Silica gel 60, F254 

Mobile Phase Ethyl acetate, methanol, water 81:11:8 (v/v/v) 

Sample Preparation Method Sample: Mix 500 mg of powdered sample with 5 mL of methanol and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions.

Derivatization reagent: Chloramine - trichloroacetic acid reagent; Preparation: Solution A: Dissolve 300 mg of sodium-N-chloro-(4-methylbenzene) sulfonamide in 10 mL of water. Solution B: Dissolve 10 g of trichloroacetic acid in ethanol and fill up to a volume of 40 mL. Mix solution A and B and shake. Use: Dip (time 0, speed 5), heat at 100°C for 8 min. 

Detection Method Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33% 

Other Notes Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.

System suitability test: Aucubin: black zone at Rf ~ 0.10 (white RT)

Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. Under white RT the chromatogram of the test solution shows a black zone at Rf ~ 0.10 corresponding to aucubin; under UV 366 nm this zone appears brown (red arrows). Under UV 366 nm there is a blue zone at Rf ~ 0.35 and a red zone due to chlorophylls right below the solvent front.

Test for adulteration: Under UV 366 nm no brown zone is seen at Rf ~ 0.10; under white RT no black zone is seen at Rf ~ 0.10 (Grecian foxglove leaf, Greater plantain leaf).

Source: HPTLC Association [6]

Supplementary Information

AHPA Logo.gif

Plantago (leaf) (Plantago lanceolata) 

General Characteristics AHPA recommends in its Known Adulterants list that appropriate steps be taken to assure that this raw material is free of the noted adulterant. Contact AHPA for additional information regarding relevant analytical methods or follow this link for more information. 

Reported Adulterants Digitalis lanata leaf.

Source: AHPA Known Adulterants [7]

Sources

  1. MOBOT, Tropicos.org http://www.tropicos.org/Image/100010935
  2. Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com
  3. Botanical Voucher Specimen Library, Alkemists Laboratories http://www.alkemist.com
  4. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  5. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  6. HPTLC Association http://www.hptlc-association.org/
  7. AHPA Known Adulterants http://www.ahpa.org/
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