Plantago ovata (seed husk)

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{{DISPLAYTITLE:''Plantago ovata'' (seed husk) }}
 
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|notes=The standard common name of the seed of P. ovata and other species of Plantago is psyllium. }}
 
|notes=The standard common name of the seed of P. ovata and other species of Plantago is psyllium. }}
  
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=Botanical Voucher Specimen=
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=Macroscopic Characteristics=
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{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
 
{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
 
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Revision as of 02:01, 16 March 2014

Contents

Nomenclature

Plantago ovata Forssk.   Plantaginaceae  
Syn. Plantago ispaghula Roxb. ex Fleming  
Standardized common name (English): Indian plantain

Botanical Voucher Specimen

Organoleptic Characteristics

Macroscopic Characteristics

Microscopic Characteristics

Epidermal cells of the husks in surface view containing mucilage observed at 400x with Acidified Chloral Hydrate Glycerol Solution.cellular structures identified in this botanical specimen are the epidermal cells of the husks in surface view containing mucilage and the parenchyma cells seen under polarized light when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [1]
AP-LOGO-Laboratories Crop - Copy.jpg
Psyllium Husk.jpg



High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Plantago ovata HPTLC ID - Vanillin/H2SO4 Reagent-> 110° C 5 min -> visible light

Psyllium (husk) (Plantago ovata)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Arabinose~0.1% in CH3OH
  2. 3 μL Plantago ovata-1 (husk)
  3. 5 μL Plantago ovata-1 (husk)
  4. 4 μL Plantago ovata-2 (husk)
  5. 4 μL Plantago ovata-2 (husk)
  6. 5 μL Plantago ovata-3 (husk)
  7. 3 μL Plantago ovata-3 (husk)
  8. 3 μL Galactose~0.1% in CH3OH


Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: glacial acetic acid: methanol: water [6/1.5/1.5/1] 

Sample Preparation Method 0.3g+3mL 70% grain EtOH sonicate/heat @~50° C ~ 1/2 hr 

Detection Method Vanillin/H2SO4 Reagent-> 110° C 5 min -> visible light 

Reference see Method Developed by Alkemists Laboratories


Source: Elan M. Sudberg, Alkemist Laboratories [2]

Supplementary Information

Sources

  1. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.Alkemist.com
  2. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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