Plantago ovata (seed husk)

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Revision as of 00:38, 27 March 2014

Contents

Nomenclature

Plantago ovata Forssk.   Plantaginaceae  
Syn. Plantago ispaghula Roxb. ex Fleming  
Standardized common name (English): Indian plantain

Botanical Voucher Specimen

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Plantago ovata Tropicos 100186592 (S).jpg
Source: MOBOT, Tropicos.org[1]

Organoleptic Characteristics

Macroscopic Characteristics

Microscopic Characteristics

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Psyllium Husk.jpg
Epidermal cells of the husks in surface view containing mucilage observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[2]

Psyllium Husk-1.jpg
Parenchyma cells seen under polarized light observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[3]


High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
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Plantago ovata HPTLC ID - Vanillin/H2SO4 Reagent-> 110° C 5 min -> visible light

Psyllium (husk) (Plantago ovata)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Arabinose~0.1% in CH3OH
  2. 3 μL Plantago ovata-1 (husk)
  3. 5 μL Plantago ovata-1 (husk)
  4. 4 μL Plantago ovata-2 (husk)
  5. 4 μL Plantago ovata-2 (husk)
  6. 5 μL Plantago ovata-3 (husk)
  7. 3 μL Plantago ovata-3 (husk)
  8. 3 μL Galactose~0.1% in CH3OH


Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: glacial acetic acid: methanol: water [6/1.5/1.5/1] 

Sample Preparation Method 0.3g+3mL 70% grain EtOH sonicate/heat @~50° C ~ 1/2 hr 

Detection Method Vanillin/H2SO4 Reagent-> 110° C 5 min -> visible light 

Reference see Method Developed by Alkemists Laboratories


Source: Elan M. Sudberg, Alkemist Laboratories [4]

Supplementary Information

Sources

  1. MOBOT, Tropicos.org http://www.tropicos.org/Image/100186592
  2. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  3. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  4. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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