Plantago ovata (seed husk)

Nomenclature

Plantago ovata Forssk.   Plantaginaceae  
Syn. Plantago ispaghula Roxb. ex Fleming  
Standardized common name (English): Indian plantain

Microscopic Characteristics

Epidermal cells of the husks in surface view containing mucilage observed at 400x with Acidified Chloral Hydrate Glycerol Solution.cellular structures identified in this botanical specimen are the epidermal cells of the husks in surface view containing mucilage and the parenchyma cells seen under polarized light when observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories

High Performance Thin Layer Chromatographic Identification

Plantago ovata HPTLC ID - Vanillin/H2SO4 Reagent-> 110° C 5 min -> visible light Psyllium (husk) (Plantago ovata) Lane Assignments Lanes, from left to right (Track, Volume, Sample): 3 μL Arabinose~0.1% in CH3OH 3 μL Plantago ovata-1 (husk) 5 μL Plantago ovata-1 (husk) 4 μL Plantago ovata-2 (husk) 4 μL Plantago ovata-2 (husk) 5 μL Plantago ovata-3 (husk) 3 μL Plantago ovata-3 (husk) 3 μL Galactose~0.1% in CH3OH Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.  Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates  Mobile Phase ethyl acetate: glacial acetic acid: methanol: water [6/1.5/1.5/1]  Sample Preparation Method 0.3g+3mL 70% grain EtOH sonicate/heat @~50° C ~ 1/2 hr  Detection Method Vanillin/H2SO4 Reagent-> 110° C 5 min -> visible light  Reference see Method Developed by Alkemists Laboratories Source: Elan M. Sudberg, Alkemist Laboratories