Triticum aestivum (leaf)

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=HPTLC Entries=
 
=HPTLC Entries=
 +
{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
 +
            | description=Wheat grass (grass) (''Triticum aestivum'')
 +
            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL=http://www.alkemist.com
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            | mainimage=Triticum aestivum - Alkemists Laboratories.jpg
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            | caption1=''Triticum aestivum'' HPTLC ID - Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm
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            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
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            | mobilephase=chloroform: methanol: water [6.4/5/1]
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            | prep=0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr
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            | detection= Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm
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            | lanes= Lanes, from left to right (Track, Volume, Sample):
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# 3 μL Aescin~0.1% in CH3OH
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# 2 μL ''Triticum aestivum''-1 (grass)
 +
# 4 μL ''Triticum aestivum''-1 (grass)
 +
# 3 μL ''Triticum aestivum''-2 (grass)
 +
# 3 μL ''Triticum aestivum''-2 (grass)
 +
# 3 μL ''Triticum aestivum''-3 (grass)
 +
# 3 μL ''Triticum aestivum''-4 (grass)
 +
# 3 μL Aescin~0.1% in CH3OH
 +
 +
Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.
 +
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            | reference= Method Developed by Alkemists Laboratories
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            | }}
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 +
 
=Other Points of Interest=
 
=Other Points of Interest=
 
[[Category:NoIntro]]
 
[[Category:NoIntro]]

Revision as of 19:24, 14 March 2013

Contents

Introduction

Macroscopic Entries

Microscopic Entries

Unicellular thick walled tapering trichome observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [1]

AP-LOGO-Laboratories Crop - Copy.jpg
Triticum aestivum - Alkemist Laboratories.jpg



HPTLC Entries

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Triticum aestivum HPTLC ID - Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm

Wheat grass (grass) (Triticum aestivum)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Aescin~0.1% in CH3OH
  2. 2 μL Triticum aestivum-1 (grass)
  3. 4 μL Triticum aestivum-1 (grass)
  4. 3 μL Triticum aestivum-2 (grass)
  5. 3 μL Triticum aestivum-2 (grass)
  6. 3 μL Triticum aestivum-3 (grass)
  7. 3 μL Triticum aestivum-4 (grass)
  8. 3 μL Aescin~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase chloroform: methanol: water [6.4/5/1] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see Method Developed by Alkemists Laboratories


Source: Elan M. Sudberg, Alkemist Laboratories [2]


Other Points of Interest


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