Triticum aestivum (leaf)

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=Introduction=
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{{DISPLAYTITLE:''Triticum aestivum'' (leaf) }} {{askbox|herb=''Triticum aestivum''}}
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=Macroscopic Entries=
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=Nomenclature=
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=Microscopic Entries=
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{{nomenclature | binomial=Triticum aestivum
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{{Microscopy | source=Elan M. Sudberg, Alkemist Laboratories
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|authority=L.
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|family=Poeceae
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|scn=wheat
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|syn=
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|ayurvedic=
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|pinyin=
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|aka=common wheat
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|notes= }}
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=Botanical Voucher Specimen=
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{{Media      |cat=Voucher
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            | source=MOBOT, Tropicos.org
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            | mainimage=Triticum aestivum Tropicos 100173767.jpg
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            | companyimage=TropicosLogo.gif
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            | companyURL=http://www.tropicos.org/Image/100173767
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            | reference=Tropicos.org. Missouri Botanical Garden. 21 Mar 2014 <http://www.tropicos.org/Image/100173767>
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            | }}
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=Organoleptic Characteristics=
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=Macroscopic Characteristics=
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=Microscopic Characteristics=
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{{Media2 |cat=Microscopy | source=Elan M. Sudberg, Alkemist Laboratories
 
             | companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
 
             | companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
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             | companyURL=http://www.Alkemist.com
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             | companyURL=http://www.alkemist.com
 
             | mainimage=Triticum aestivum - Alkemist Laboratories.jpg
 
             | mainimage=Triticum aestivum - Alkemist Laboratories.jpg
 
             | caption1=Unicellular thick walled tapering trichome observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
 
             | caption1=Unicellular thick walled tapering trichome observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
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| source2=Elan M. Sudberg, Alkemist Laboratories
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            | companyimage2= AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL2=http://www.alkemist.com
 
             | description= Wheat Grass (leaf) (''Triticum aestivum'')
 
             | description= Wheat Grass (leaf) (''Triticum aestivum'')
 
             | image2=Triticum aestivum-1 - Alkemist Laboratories.jpg
 
             | image2=Triticum aestivum-1 - Alkemist Laboratories.jpg
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=HPTLC Entries=
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=High Performance Thin Layer Chromatographic Identification=
 
{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
 
{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
 
             | description=Wheat grass (grass) (''Triticum aestivum'')
 
             | description=Wheat grass (grass) (''Triticum aestivum'')
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             | }}
 
             | }}
  
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{{HPTLC | source=HPTLC Association
 
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            | companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
 
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            | companyURL=http://www.hptlc-association.org/
 
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            | mainimage=Curcuma longa w nim-UV 254 nm-hptlc-association.png
 
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            | caption1=Turmeric adulteration with nimesulide (rhizome) HPTLC ID - UV 254 nm
 
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            | description=Turmeric adulteration with nimesulide (rhizome) (''Curcuma longa'')
 
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            | image2=Curcuma longa w nim-2,5-Dichloro-1,4-benzoquinone reagent, UV 366 nm-hptlc-association.png
 
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            | caption2=Turmeric adulteration with nimesulide (rhizome) HPTLC ID - 2,5-Dichloro-1,4-benzoquinone reagent, UV 366 nm
 
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            | image3=Curcuma longa w nim-2,5-Dichloro-1,4-benzoquinone reagent, white RT-hptlc-association.png
 
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            | caption3=Turmeric adulteration with nimesulide (rhizome) HPTLC ID - 2,5-Dichloro-1,4-benzoquinone reagent, white RT
 
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            |
 
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            | stationaryphase=Stationary phase, i.e. Silica gel 60, F254
 
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            | mobilephase=Toluene, acetic acid (4:1) (v/v)
 
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            | prep=Sample: Mix 0.2 g of powdered sample with 3 mL of methanol, sonicate for 10 minutes, then centrifuge or filter the solution and use the supernatant/filtrate as test solution.
 
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Derivatization reagent: 2,5-Dichloro-1,4-benzoquinone reagent, Preparation: 0.50 g of 2,5-Dichloro-1,4-benzoquinone is dissolved in 80 mL of dimethyl sulfoxide and then diluted with 160 mL of tetrahydrofuran, Use: Dip (time 0, speed 5), dry in a stream of cold air for 3 min.
 
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            | detection=Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33%
 
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            | referencesamples=Reference: Dissolve 1.0 mg of nimesulide in 10 mL of methanol.
 
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            | lanes=Lanes, from left to right (Track, Volume, Sample):
 
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# 2 µL Turmeric 1 (not spiked)
 
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# 2 µL Turmeric 1 (spiked w/ 1% nimesulide)
 
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# 2 µL Turmeric 1 (w/ 2% nimesulide)
 
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# 2 µL Turmeric 1 (w/ 5% nimesulide)
 
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# 2 µL Turmeric 1 (w/ 10% nimesulide)
 
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# 2 µL Turmeric 2 (not spiked)
 
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# 2 µL Turmeric 2 (w/ 1% nimesulide)
 
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# 2 µL Turmeric 2 (w/ 2% nimesulide)
 
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# 2 µL Turmeric 2 (w/ 5% nimesulide)
 
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# 2 µL Turmeric 2 (w/ 10% nimesulide)
 
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# 2 µL Turmeric 3 (not spiked)
 
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# 2 µL Turmeric 3 (w/ 1% nimesulide)
 
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# 2 µL Turmeric 3 (w/ 2% nimesulide)
 
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# 2 µL Turmeric 3 (w/ 5% nimesulide)
 
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# 2 µL Turmeric 3 ( w/ 10% nimesulide)
 
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            | notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.''
 
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System suitability test: Nimesulide: Rf ~  0.51
 
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Test for adulteration: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows no zone at Rf ~ 0.51 corresponding in color and position to that of the nimesulide reference standard.
 
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            | }}
 
  
  
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=Other Points of Interest=
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=Supplementary Information=
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[[Category:NoIntro]]
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=Sources=
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<references />

Latest revision as of 21:25, 2 June 2014

AHPA recognizes other valuable resources exist regarding the identity of Triticum aestivum.

To submit a suggestion or contribution, please contact Merle Zimmermann.

Contents

Nomenclature

Triticum aestivum L.   Poeceae  
Standardized common name (English): wheat

Botanical Voucher Specimen

bottomright

Triticum aestivum Tropicos 100173767.jpg
Source: MOBOT, Tropicos.org[1]

Organoleptic Characteristics

Macroscopic Characteristics

Microscopic Characteristics

bottomright bottomright

Triticum aestivum - Alkemist Laboratories.jpg
Unicellular thick walled tapering trichome observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[2]

Triticum aestivum-1 - Alkemist Laboratories.jpg
Epidermis showing long thin stomata observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[3]


High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Triticum aestivum HPTLC ID - Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm

Wheat grass (grass) (Triticum aestivum)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Aescin~0.1% in CH3OH
  2. 2 μL Triticum aestivum-1 (grass)
  3. 4 μL Triticum aestivum-1 (grass)
  4. 3 μL Triticum aestivum-2 (grass)
  5. 3 μL Triticum aestivum-2 (grass)
  6. 3 μL Triticum aestivum-3 (grass)
  7. 3 μL Triticum aestivum-4 (grass)
  8. 3 μL Aescin~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase chloroform: methanol: water [6.4/5/1] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see Method Developed by Alkemists Laboratories


Source: Elan M. Sudberg, Alkemist Laboratories [4]


Supplementary Information

Sources

  1. ↑ MOBOT, Tropicos.org http://www.tropicos.org/Image/100173767
  2. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  3. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  4. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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