Vitis vinifera (seed)

From AHPA Botanical Identity References Compendium
(Difference between revisions)
Jump to: navigation, search
(add askbox)
 
(11 intermediate revisions by 2 users not shown)
Line 1: Line 1:
−
=Introduction=
+
{{DISPLAYTITLE:''Vitis vinifera'' (seed) }} {{askbox|herb=''Vitis vinifera''}}
−
=Macroscopic Entries=
+
=Nomenclature=
 +
{{nomenclature | binomial=Vitis vinifera
 +
|authority=L.
 +
|family=Vitaceae
 +
|scn=grape
 +
|syn=
 +
|ayurvedic=draksha
 +
|pinyin=
 +
|aka=European grape; wine grape
 +
|notes= }}
  
−
=Microscopic Entries=
+
=Botanical Voucher Specimen=
−
{{Microscopy | source=Elan M. Sudberg, Alkemist Laboratories-American Herbal Pharmacopoeia & Therapeutic Compendium
+
 
 +
{{Media      | cat=Voucher
 +
 
 +
            | source=MOBOT, Tropicos.org
 +
            | mainimage=Vitis_vinifera_Tropicos_100172793_(S).jpg
 +
            | companyimage=TropicosLogo.gif
 +
            | companyURL=http://www.tropicos.org/Image/100172793
 +
            | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100172793>
 +
 
 +
            | }}
 +
 
 +
=Organoleptic Characteristics=
 +
 
 +
=Macroscopic Characteristics=
 +
=Microscopic Characteristics=
 +
{{Media2 |cat=Microscopy | source=Elan M. Sudberg, Alkemist Laboratories
 
             | companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
 
             | companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
−
             | companyURL=http://www.Alkemist.com
+
             | companyURL=http://www.alkemist.com
 
             | mainimage=Vitis vinifera seed.jpg
 
             | mainimage=Vitis vinifera seed.jpg
 
             | caption1=Raphides containing idioblast of needles of calcium oxalate observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
 
             | caption1=Raphides containing idioblast of needles of calcium oxalate observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
−
             | description= Grape (seed) (''Vitis vinifera'')
+
|source2=Elan M. Sudberg, Alkemist Laboratories
 +
            | companyimage2= AP-LOGO-Laboratories Crop - Copy.jpg
 +
             | companyURL2=http://www.alkemist.com
 
             | image2=Vitis vinifera seed-1.jpg
 
             | image2=Vitis vinifera seed-1.jpg
 
             | caption2=Thick layer of sclereids observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
 
             | caption2=Thick layer of sclereids observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
 
             | characteristics=cellular structures identified in this botanical specimen are the raphides containing idioblast of needles of calcium oxalate and the thick layer of sclereids when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
 
             | characteristics=cellular structures identified in this botanical specimen are the raphides containing idioblast of needles of calcium oxalate and the thick layer of sclereids when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
 +
            | reference=American Herbal Pharmacopoeia & Therapeutic Compendium
 +
            | }}
 +
 +
=High Performance Thin Layer Chromatographic Identification=
 +
{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
 +
            | description=Grape (seed) (''Vitis vinifera'')
 +
            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
 +
            | companyURL=http://www.alkemist.com
 +
            | mainimage=Vitis_vinifera_-_Alkemists_Laboratories.jpg
 +
            | caption1=''Vitis vinifera'' HPTLC ID - Vanillin Sulfuric Reagent UV 365 nm
 +
            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
 +
            | mobilephase=chloroform: ethyl formate: formic acid [6/3/1]
 +
            | prep=0.3 g + 3ml 70% grain EtOH sonicated + heated @ 50° C ~ 1 hr
 +
            | detection=Vanillin/H2SO4 spray reagent -> 110° C 5 min -> UV 365 nm
 +
            | lanes= Lanes, from left to right (Track, Volume, Sample):
 +
# 3 μL Catechin ~0.1% in Methanol
 +
# 3 μL ''Vitis vinifera''-1 (peel)
 +
# 3 μL ''Vitis vinifera''-2 (seed)
 +
# 3 μL ''Vitis vinifera''-3 (seed)
 +
# 3 μL ''Vitis vinifera''-3 (seed)
 +
# 3 μL ''Vitis vinifera''-4 (seed)
 +
# 3 μL ''Vitis vinifera''-5 (seed)
 +
# 3 μL Epicatechin gallate ~0.1% in Methanol
 +
 +
Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.
 +
 +
            | reference=American Herbal Pharmacopoeia & Therapeutic Compendium
 
             | }}
 
             | }}
  
−
=HPTLC Entries=
+
=Supplementary Information=
−
=Other Points of Interest=
+
=Sources=
 +
<references />

Latest revision as of 21:30, 2 June 2014

AHPA recognizes other valuable resources exist regarding the identity of Vitis vinifera.

To submit a suggestion or contribution, please contact Merle Zimmermann.

Contents

Nomenclature

Vitis vinifera L.   Vitaceae  
Standardized common name (English): grape  
Ayurvedic name(s): draksha

Botanical Voucher Specimen

bottomright

Vitis vinifera Tropicos 100172793 (S).jpg
Source: MOBOT, Tropicos.org[1]

Organoleptic Characteristics

Macroscopic Characteristics

Microscopic Characteristics

bottomright bottomright

Vitis vinifera seed.jpg
Raphides containing idioblast of needles of calcium oxalate observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[2]

Vitis vinifera seed-1.jpg
Thick layer of sclereids observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[3]

High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Vitis vinifera HPTLC ID - Vanillin Sulfuric Reagent UV 365 nm

Grape (seed) (Vitis vinifera)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Catechin ~0.1% in Methanol
  2. 3 μL Vitis vinifera-1 (peel)
  3. 3 μL Vitis vinifera-2 (seed)
  4. 3 μL Vitis vinifera-3 (seed)
  5. 3 μL Vitis vinifera-3 (seed)
  6. 3 μL Vitis vinifera-4 (seed)
  7. 3 μL Vitis vinifera-5 (seed)
  8. 3 μL Epicatechin gallate ~0.1% in Methanol

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase chloroform: ethyl formate: formic acid [6/3/1] 

Sample Preparation Method 0.3 g + 3ml 70% grain EtOH sonicated + heated @ 50° C ~ 1 hr 

Detection Method Vanillin/H2SO4 spray reagent -> 110° C 5 min -> UV 365 nm 

Reference see American Herbal Pharmacopoeia & Therapeutic Compendium


Source: Elan M. Sudberg, Alkemist Laboratories [4]

Supplementary Information

Sources

  1. ↑ MOBOT, Tropicos.org http://www.tropicos.org/Image/100172793
  2. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  3. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  4. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
Retrieved from "http://www.botanicalauthentication.org/index.php?title=Vitis_vinifera_(seed)&oldid=6544"
MediaWiki Appliance - Powered by TurnKey Linux