Vitis vinifera HPTLC ID - Vanillin Sulfuric Reagent UV 365 nm

Grape (seed) (Vitis vinifera)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

1. 3 μL Catechin ~0.1% in Methanol
2. 3 μL Vitis vinifera-1 (peel)
3. 3 μL Vitis vinifera-2 (seed)
4. 3 μL Vitis vinifera-3 (seed)
5. 3 μL Vitis vinifera-3 (seed)
6. 3 μL Vitis vinifera-4 (seed)
7. 3 μL Vitis vinifera-5 (seed)
8. 3 μL Epicatechin gallate ~0.1% in Methanol

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase chloroform: ethyl formate: formic acid [6/3/1] 

Sample Preparation Method 0.3 g + 3ml 70% grain EtOH sonicated + heated @ 50° C ~ 1 hr 

Detection Method Vanillin/H2SO4 spray reagent -> 110° C 5 min -> UV 365 nm 

Reference see American Herbal Pharmacopoeia & Therapeutic Compendium

Source: Elan M. Sudberg, Alkemist Laboratories ^[2]