Vitis vinifera (seed)

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{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
 
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Revision as of 03:08, 16 March 2014

Contents

Nomenclature

Vitis vinifera L.   Vitaceae  
Standardized common name (English): grape  
Ayurvedic name(s): draksha

Botanical Voucher Specimen

Organoleptic Characteristics

Macroscopic Characteristics

Microscopic Characteristics

Raphides containing idioblast of needles of calcium oxalate observed at 400x with Acidified Chloral Hydrate Glycerol Solution.cellular structures identified in this botanical specimen are the raphides containing idioblast of needles of calcium oxalate and the thick layer of sclereids when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [1]

AP-LOGO-Laboratories Crop - Copy.jpg
Vitis vinifera seed.jpg


High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Vitis vinifera HPTLC ID - Vanillin Sulfuric Reagent UV 365 nm

Grape (seed) (Vitis vinifera)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Catechin ~0.1% in Methanol
  2. 3 μL Vitis vinifera-1 (peel)
  3. 3 μL Vitis vinifera-2 (seed)
  4. 3 μL Vitis vinifera-3 (seed)
  5. 3 μL Vitis vinifera-3 (seed)
  6. 3 μL Vitis vinifera-4 (seed)
  7. 3 μL Vitis vinifera-5 (seed)
  8. 3 μL Epicatechin gallate ~0.1% in Methanol

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase chloroform: ethyl formate: formic acid [6/3/1] 

Sample Preparation Method 0.3 g + 3ml 70% grain EtOH sonicated + heated @ 50° C ~ 1 hr 

Detection Method Vanillin/H2SO4 spray reagent -> 110° C 5 min -> UV 365 nm 

Reference see American Herbal Pharmacopoeia & Therapeutic Compendium


Source: Elan M. Sudberg, Alkemist Laboratories [2]

Supplementary Information

Sources

  1. Elan M. Sudberg, Alkemist Laboratories http://www.Alkemist.com
  2. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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