Vitis vinifera (seed)

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=Botanical Voucher Specimen=
 
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=Organoleptic Characteristics=
 
=Organoleptic Characteristics=
  

Revision as of 03:43, 27 March 2014

Contents

Nomenclature

Vitis vinifera L.   Vitaceae  
Standardized common name (English): grape  
Ayurvedic name(s): draksha

Botanical Voucher Specimen

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Vitis vinifera Tropicos 100172793 (S).jpg
Source: MOBOT, Tropicos.org[1]

Organoleptic Characteristics

Macroscopic Characteristics

Microscopic Characteristics

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Vitis vinifera seed.jpg
Raphides containing idioblast of needles of calcium oxalate observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[2]

Vitis vinifera seed-1.jpg
Thick layer of sclereids observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[3]

High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Vitis vinifera HPTLC ID - Vanillin Sulfuric Reagent UV 365 nm

Grape (seed) (Vitis vinifera)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Catechin ~0.1% in Methanol
  2. 3 μL Vitis vinifera-1 (peel)
  3. 3 μL Vitis vinifera-2 (seed)
  4. 3 μL Vitis vinifera-3 (seed)
  5. 3 μL Vitis vinifera-3 (seed)
  6. 3 μL Vitis vinifera-4 (seed)
  7. 3 μL Vitis vinifera-5 (seed)
  8. 3 μL Epicatechin gallate ~0.1% in Methanol

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase chloroform: ethyl formate: formic acid [6/3/1] 

Sample Preparation Method 0.3 g + 3ml 70% grain EtOH sonicated + heated @ 50° C ~ 1 hr 

Detection Method Vanillin/H2SO4 spray reagent -> 110° C 5 min -> UV 365 nm 

Reference see American Herbal Pharmacopoeia & Therapeutic Compendium


Source: Elan M. Sudberg, Alkemist Laboratories [4]

Supplementary Information

Sources

  1. MOBOT, Tropicos.org http://www.tropicos.org/Image/100172793
  2. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  3. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  4. Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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