Vitis vinifera (seed)

Nomenclature

Vitis vinifera L.   Vitaceae  
Standardized common name (English): grape  
Ayurvedic name(s): draksha

Microscopic Characteristics

Raphides containing idioblast of needles of calcium oxalate observed at 400x with Acidified Chloral Hydrate Glycerol Solution.cellular structures identified in this botanical specimen are the raphides containing idioblast of needles of calcium oxalate and the thick layer of sclereids when observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories

High Performance Thin Layer Chromatographic Identification

Vitis vinifera HPTLC ID - Vanillin Sulfuric Reagent UV 365 nm Grape (seed) (Vitis vinifera) Lane Assignments Lanes, from left to right (Track, Volume, Sample): 3 μL Catechin ~0.1% in Methanol 3 μL Vitis vinifera-1 (peel) 3 μL Vitis vinifera-2 (seed) 3 μL Vitis vinifera-3 (seed) 3 μL Vitis vinifera-3 (seed) 3 μL Vitis vinifera-4 (seed) 3 μL Vitis vinifera-5 (seed) 3 μL Epicatechin gallate ~0.1% in Methanol Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.  Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates  Mobile Phase chloroform: ethyl formate: formic acid [6/3/1]  Sample Preparation Method 0.3 g + 3ml 70% grain EtOH sonicated + heated @ 50° C ~ 1 hr  Detection Method Vanillin/H2SO4 spray reagent -> 110° C 5 min -> UV 365 nm  Reference see American Herbal Pharmacopoeia & Therapeutic Compendium Source: Elan M. Sudberg, Alkemist Laboratories